Primary neuronal cultures are indispensable in the field of neurobiology and pharmacology. Many researchers favor freshly isolated neuronal cells as they maintain their functional viability, but for convenience, an alternate route is to cryopreserve fresh cells for later use. This chapter describes the generation of cryopreserved stocks from the freshly isolated neural cells, and thawing procedures for recovering the stocks. Handle cells gently, because they are extremely fragile upon recovery from cryopreservation. Do not centrifuge cells upon recovery from cryopreservation.
Cryopreservation and Recovery of Mature Differentiated Neural Cells | Thermo Fisher Scientific - FR
The objective of this study was to determine the mode of inheritance of field resistance to downy mildew Peronospora parasitica Pers. The F 1 , F 2 and F 3 progeny of resistant and susceptible plants of broccoli were tested in the field under natural infection, in central Portugal, from August to December in two successive years. The plants were evaluated for resistance to downy mildew at maturity using a five-class scale of increasing susceptibility to the disease, which took into account the number of infected leaves and the size of the sporulating lesions. The F 1 was completely resistant, the F 2 segregated a clear 3 resistant: 1susceptible and the F 3 confirmed the F 2 segregation, which suggests a dominant character controlled by a single locus. This resistance has good potencial for direct use in commercial broccoli breeding or for transfer to other Brassica vegetables. This is a preview of subscription content, access via your institution.
Mature, but different: Best practices for the Australian market
Exosomes are vesicles released by most cells into their environment upon fusion of multivesicular endosomes with the plasma membrane. We use dissociated cortical neurons cultured for around two weeks as exosome-releasing cells. In these conditions, neurons make mature synapses and form networks that can be activated by physiological stimuli.
When the Universe was only a tenth of its current age its galaxies experienced a growth spurt. To their surprise, these galaxies observed in the early stages of their life were far more mature than expected. Galaxies began to form very early in the history of the Universe. To study their infancy, it is therefore necessary to go back to the dawn of time, by observing very distant galaxies.